1/3/2024 0 Comments Aplf clover configuratorA cell library for use in detecting protein interactionsīetween a protein of interest and a set of target proteins, said The library were generated from cells obtained from a transgenicĪnimal configured to express a CRISPR enzyme.ġ6. The library according to claim 13 or 14, wherein the cells of Inducible or transiently delivered as a protein.ġ5. The library according to claim 13, wherein the CRISPR enzyme is The library according to any of claims 1 to 12, wherein theĬells of the library were generated from cells configured toġ4. The library according to claim 11, wherein the eukaryotic cellsĪre mammalian, insect, yeast, or plant cells.ġ3. The library according to any of claims 1 to 10, wherein theġ2. The tagged target gene, whereby detection of the sequence indicatesġ1. The library according to any of claims 1 to 9, wherein eachĬell comprises a sequence encoding a guide sequence specific for The library according to any of claims 1 to 8, wherein theġ0. Localization signal, lysosome localization signal, or mitochondrialĩ. Peroxisome localization signal, ER localization signal, golgi The library according to claim 7, wherein the protease comprisesĪt least one nuclear export signal, nuclear localization signal, The library according to claim 6, wherein each cell isĬonfigured for expression of a recombinant protease localized to aĨ. Polynucleotide sequence encoding a detectable marker furtherĬomprises a sequence encoding a protease cleavage site and aĬleavable marker, wherein the sequence is in frame with theħ. The library according to any of claims 1 to 5, wherein the The library according to claim 3, wherein the selectable markerĦ. The selectable marker is expressed as a separate protein.ĥ. Polynucleotide sequence comprises an IRES or a 2A peptide, whereby Is operably linked to a separate regulatory element or wherein the The library according to claim 3, wherein the selectable marker Polynucleotide sequence further comprises a selectable markerĤ. The library according to claim 1 or 2, wherein the Sequence-verified, such that each UMI identifies a tagged targetģ. Marker coding sequence, preferably, wherein the library is Identifier (UMI) sequence or a non-coding UMI after the detectable Sequence further comprises a codon-neutral unique molecular The library according to claim 1, wherein the polynucleotide Levels in single cells comprising a plurality of cells, whereinĮach cell comprises a polynucleotide sequence encoding a detectableĢ. A cell library for use in detecting the distribution of protein This invention was made with government support under grant STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH Population of cells and for performing proteomics studies. Proteins in a cell and kits comprising vectors for tagging a Also provided are systems for analysis of Sequencing integration sites of a donor sequence inserted into the The invention also provides methods of constructingĪ cell library for use in proteomics, as well as methods for Proteins and a cell library for use in detecting protein Interactions between a protein of interest and a set of target Gene, as well as a cell library for use in detecting protein The library comprises more than one cell tagged at each target Protein coding gene selected from a set of target genes, wherein Marker integrated into the genome of the cell in frame with a Protein expression comprising a plurality of cells, wherein eachĬell comprises a polynucleotide sequence encoding a detectable The invention provides a cell library for use in detecting Invention is credited to Veit Hornung, Jonathan Leo Schmid-Burgk, Feng Zhang. The applicant listed for this patent is THE BROAD INSTITUTE, INC., MASSACHUSETTS INSTITUTE OF TECHNOLOGY. This patent application is currently assigned to THE BROAD INSTITUTE, INC. patent application number 17/048369 was filed with the patent office on for sequencing-based proteomics.
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